The instruments measure electrical impedance across interdigitated micro-electrodes integrated on the bottom of its special tissue culture plates. Cell growth behavior was continuously monitored for 48 hours using the xCELLigence RTCA MP Instrument (Roche Diagnostics GmbH, Mannheim, Germany). The xCELLigence RTCA MP Instrument (ACEA Biosciences) was used for measuring the T‐cell killing of the BKV antigen‐expressed cells. Computer-controlled signal generation and automatic frequency scanning enable the continual precise detection of changes in cell behaviour using 96-well culture plates. HeLa cells were seeded at 10,000 cells per well in an E-Plate 96 and grown in the xCELLigence RTCA MP Instrument placed in an incubator overnight. To maximize the throughput, up to four instruments can be integrated and monitored by one control unit. RTCA SP Instrument Highlights: Rapid measurement time - less than 10 or 60 seconds for 96 wells; Compact design of the RTCA SP Station that fits conveniently in tissue culture incubators; ... brochure_sp-mp (1 007 kB) xcelligence (4,22 MB) Discover more. The RTCA MP has a higher throughput capacity than the RTCA SP and is more versatile, enabling application on a wider range of experimental set-ups. The xCELLigence MP Instrument uses proprietary software and E-Plates 96 to measure electronic cell impedance using sensor electrodes. I love it! " After anniversary update, mtp is not working. Monitoring of Cell Growth With the RTCA MP Instrument. After seeding the BSMCs adhere to the E-plate and begin to spread and proliferate, which are recorded as changes of impedance that are reported as Cell Index (CI) by xCELLigence (Fig. PENZBERG, Germany--(BUSINESS WIRE)--Following the launch of the xCELLigence RTCA SP Instrument in June, Roche Applied Science has introduced the new xCELLigence RTCA MP Instrument. These biosensors continuously and non-invasively monitor changes in cell number, cell size, cell barrier function, and cell-substrate attachment quality. Agilent ACEA xCELLigence RTCA MP W380 Real-Time Cell AnalyzerInternal part number 18079Here is an Agilent ACEA xCELLigence RTCA MP W380 Real-Time Cell Analyzer This allows for high-throughput screening of drugs and compounds. ACHP substantially suppressed the proliferation of A549, compared with the untreated cells, as shown in Figure 3 A. Interestingly, ACHP decreased the IL-6 induced proliferation of H1299 cells. First, 50 uL of target cancer cell culturing media was added to each well of 96 well E-Plates (ACEA Biosciences) and the background impedance was measured and displayed as Cell Index[ 16 , 19 , … Review date: 15 Jun 2020 | xCELLigence RTCA eSight™ xCELLigence - RTCA MP - Multiple Plates Real Time Cell Analyzer by ACEA Biosciences, Inc.. Growth media were then removed, assay buffer added, and after 15 minutes of continuous cell monitoring, compounds that … The Xcelligence system measures impedance-based signals both in label-free cellular analysis and in cell invasion/migration assays. • xCELLigence RTCA SP or MP instruments: While all of the xCELLigence RTCA instruments can be used for this liquid tumor killing assay, the cell numbers and . It offers 5 separate cradles for more flexibility. The cancer cells were incubated with ACHP (10 µM) and viability was measured at intervals of 15 min using the xCELLigence RTCA MP Instrument (Roche Diagnostics GmbH, Germany). Instrument Prep and Measuring Background Impedance Experiments were conducted using an xCELLigence® RTCA MP (multi-plate) instrument (cat.# 00380601040) that was housed in a standard tissue culture incubator set to maintain 37ºC. It´s technology is compatible with workflows carrying out automated screening using the 384-wel more Instrument Plate Type / Format Throughput / Samples per Run Applicati ons RTCA SP Instrument (1) E-Plate 96 or E-Plate VIEW 96 96 • Cellular Assays xCELLigence ... cell adhesion and migration. In comparison with the RTCA SP, the new RTCA MP Instrument has a higher throughput capacity and is versatile which enables application on a wider range of experimental set-ups. Roche’s xCELLigence System RTCA HT Instrument performs real-time impedance-based label-free measurement of cell behavior for high throughput applications. In comparison with the RTCA SP, the new RTCA MP Instrument has a higher throughput capacity and it is more versatile which enables application on a wider range of experimental set-ups. This gives you a maximal screening capacity of 1536 wells in total. ST‐HEK293 cells were first seeded at a density of 60 000 cells per well of the E‐plate to a total volume of 150 μL with DMEM–10% FCS media. We tested the xCELLigence RTCA MP instrument with 96-well E-plate, which is normally used to monitor cell attachment and growth in real time. ThexCELLigence RTCA MP Instrumentenables more cellular analysis than ever before. " This instrument combines impedance measurement with imaging in 3 colors plus bright field. Easy-to-use intuitive software allows for real-time control and monitoring of the instrument and includes real-time data display and analysis functions. Background impedance was measured in 100 µL cell culture medium per well. The RTCA SP instrument holds one 96-well E-plate whereas the RTCA MP instrument can hold up to six 96-well E-plates simultaneously. The xCELLigence instrument is manufactured in three different configurations: real time cell analyzer (RTCA) SP, RTCA MP and RTCA DP. To learn more about our new Real-Time Cell Analyzer, visit: www.xcelligence.roche.com Neverletacelleventgoundetected xCELLigence RTCA MP Instrument www.roche-applied-science.com Roche Applied Science has introduced ACEA Biosciences' Xcelligence RTCA MP Instrument, which needs less than 15 seconds to analyse a 96-well E Plate. Although these instructions have been optimized using the xCELLigence RTCA S16 (Pilot Scale) xCELLigence RTCA DP (Cell Invasion & Migration) xCELLigence RTCA SP (Single Plate) xCELLigence RTCA MP (Multiple Plates) xCELLigence RTCA HT (High Throughput Model) xCELLigence RTCA Cardio (Cardiomyocyte Contractility) xCELLigence RTCA CardioECR (Cardiomyocyte Contractility + Electrical Activity) • ®xCELLigence® RTCA SP or MP instruments: While all of the xCELLigence RTCA instruments can be used for this B cell killing assay, the cell numbers and media volumes listed in this protocol are optimized for the dimensions of the wells in the E-Plate® View 96 – which is only compatible with the xCELLigence® RTCA SP and MP instruments. The xCELLigence RTCA MP instrument (ACEA Biosciences) was utilized for all impedance experiments. Higher throughput for xCELLigence cell based assay. As of 2020, it is used by dozens of universities and the united states department of agriculture. Not for use in diagnostic procedures. Note that other Agilent xCELLigence instruments (RTCA multiple plates (MP), dual purpose (DP), single 16-well plate (S16), and high throughput (HT)) may also be used. Housing the xCELLigence® instrument in a hypoxia chamber or in different atmospheric compositions (CO 2 The RTCA MP Station is located inside a tissue culture incubator and is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement.Each of the six E-Plate holders can be used independently under the RTCA Software. Cancer Immunotherapy Applications of xCELLigence® RTCA Systems 3 Overiew What is the xCELLigence RTCA assay principle? The IAC is equipped with the xCELLigence RTCA MP System and the xCELLigence RTCA DP System. Xcelligence MP aids human kinome RNAi study | Laboratory Talk The Xcelligence MP instrument uses proprietary software and E-Plates 96 to measure electronic cell impedance using sensor electrodes. XCELLigence RTCA MP Multi Plate Instrument, ACEA. The MP (multiple plate) model can host up to six 96-well E-Plates. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. The xCELLigence® RTCA Cardio instrument expands upon ACEA’s microelectronic-based cellular assay systems to enable monitoring of cardiomyocyte beating for cardiotoxicity assessment. The RTCA DP (dual plate) instrument is applicable for flexible low-throughput research assays and uses up to three 16-well plates for simultaneous experiments. xCELLigence MP - Acea Biosciences. The Agilent xCELLigence RTCA MP instrument monitors immune cell killing in a label-free, real-time manner. xCELLigence RTCA MP Instrument RTCA Analyzer RTCA MP Station RTCA Control Unit 00380601040 05228972001 05331625001 05454417001 1 Bundled Package 1 Instrument 1 Instrument 1 Notebook PC E-Plate 16 E-Plate VIEW 16 E-Plate Insert 16 05469830001 05469813001 06324738001 06324746001 06465382001 6 Plates 6 x 6 Plates The xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize patented microtiter plates (E-Plates®) which contain gold biosensors embedded in the bottom of each well. Cardiomyocyte contractility and viability measured in real-time. The xCELLigence RTCA MP instrument (ACEA Biosciences) was utilized for all impedance experiments. Plus it's easy to use! The xCELLigence system (Real-Time Cell Analyzer RTCA-SP, ACEA Biosciences, San Diego, USA) was used for impedance-based real-time detection of cell viability (Ke et al., 2011). 7 media volumes listed in this protocol are optimized for the dimensions of the ACEA’s xCELLigence® Real-Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor cell status including cell number, shape/size and attachment. RTCA SP Instrument RTCA DP Instrument RTCA MP Instrument RTCA HT Instrument RTCA Cardio Instrument For life science research only. The xCELLigence ® RTCA MP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner. The MP (multiple plate) model differs from our other xCELLigence ® instruments in that it can host up to six 96-well electronic microtiter plates (E-Plate ® 96). 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